Assume the two E.coli strains shown below are allowed to conjugate.

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Assume the two E.coli strains shown below are allowed to conjugate.
Hfr: pro+, arg+, his+, lys+, met+, ampicillin-sensitive
F-: pro-, arg-, his-, lys-, met-, ampicillin-resistant
What supplements would you add to glucose minimal salts agar to select for a recombinant cell that is lys+, arg+, amp-resistant?
-ampicillin, lysine, arginine
-ampicillin, proline, histidine, lysine
-lysine, arginine
-proline, histidine, methionine
-ampicillin, proline, histidine, methionine

The Correct Answer is

ampicillin, proline, histidine, methionine

Reason Explained

ampicillin, proline, histidine, methionine is correct for Assume the two E.coli strains shown below are allowed to conjugate.

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